ros fluorescence assay kit  (Elabscience Biotechnology)

Journal: Frontiers in Pharmacology

Article Title: Pemetrexed sensitizes cisplatin therapy by inducing ferroptosis in NSCLC cells

doi: 10.3389/fphar.2025.1764937

Figure Lengend Snippet: PEM induces ferroptosis in cells. A549 cells were grown with or without PEM (100 nM) for 72 h. (A) Heatmap summarizing RNA-seq data for the control and PEM-treated A549 cells with 744 upregulated genes (Red) and 412 downregulated genes (Green). (B) Volcano plot of differentially expressed genes between the control and PEM-treated cells as determined by RNA-seq. (C) KEGG enrichment analysis of PEM-regulated genes. The A549 or H1299 cells were subjected to PEM (100 nM), or DDP (200 nM), or PEM (100 nM) and DDP (200 nM) for 72 h. (D) The intracellular ROS was tested by flow cytometry, and the quantification of fluorescence intensities is shown. The levels of GSH (E) , SOD (F) , MDA (G) , and intercellular iron (H) were measured using appropriate kits, and the corresponding statistical results are shown. Western blot was used to detect (I) the expression of anti-ferroptosis proteins (SLC7A11, GPX4, DHODH, FSP1, and GCH1) and (J) FPN1, FTH1, FTL, and (K) the expression of pro-ferroptosis proteins (ACSL4, 12LOX, COX2, DMT1, TFR1, and TF) in A549 cells. ( * P < 0.01; ** P < 0.01; *** P < 0.005; **** P < 0.001).

Article Snippet: Intracellular ROS fluorescence assay kits (WLA131; Wanleibio, China) were used to measure the levels of ROS according to the kit instructions.

Techniques: RNA Sequencing, Control, Flow Cytometry, Fluorescence, Western Blot, Expressing